|
KMID : 0370019950090000067
|
|
Chung-Ang Journal of Pharmacal Sciences
1995 Volume.9 No. 0 p.67 ~ p.74
|
|
|
Induction of cell growth inhibition and apoptosis by ceramide
|
|
윤기호/Yoon, Ki Ho
최관수/이강균/김미영/Choi, Kwan Soo/Lee, Kang Kyun/Kim, Mie Young
|
|
|
Abstract
|
|
|
|
Sphingolipids play important roles in cell regulation and signal transduction. Recently, a sphingomyelin cycle has been described in which activation of neutral sphingomyelinase leads to the breakdown of sphingomyelin and the generation of ceramide. Ceramide, in turn, has emerged as a candidate intracellular mediator for action of certain cell agonists and has multiple biologic actions. Ceramide is potent suppressor of cell growth and an inducer of apoptosis. The present studies show that exposure of IM-9 cells to ceramide resulted in internucleosomal cleavage of DNA. DNA fragmentation induced by ceramide was confirmed by ^3H-thymidine release and diphenylamine assay. Transmission electron micrographs of IM-9 cells treated with ceramide showed typical apoptotic morphology with marked nuclear chromatin condensation and apoptotic bodies. These results show a role for ceramide in the regulation of cell growth and apoptosis.
|
|
|
KEYWORD
|
|
|
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|